World Journal of Microbiology and Biotechnology

, Volume 12, Issue 6, pp 615–617

Characteristics of extracellular proteases produced by Bacillus laterosporus and Flavobacterium sp. isolated from gelatinfactory effluents

  • A. Sharma
  • C. L. S. N. Rao
  • B. K. Ball
  • S. K. Hasija
Research

DOI: 10.1007/BF00327724

Cite this article as:
Sharma, A., Rao, C.L.S.N., Ball, B.K. et al. World Journal of Microbiology & Biotechnology (1996) 12: 615. doi:10.1007/BF00327724

Abstract

Forty bacterial isolates from the effluents of a gelatin factory (Jabalpur, India) were screened for protease activity and the two most potent producers were identified as Bacillus laterosporus and a Flavobacterium sp. The enzymes of both isolates were optimal at pH 8 and 60°C, with maximum activity after 90 min. The enzyme activity of B. laterosporus was suppressed by Fe2+, Mg2+, Mn2+ and Zn2+ ions but was enhanced by Ba2+ and Ca2+. That of Flavobacterium sp. was suppressed by Mg2+ and Mn2+ ions but enhanced by Ba2+, Ca2+ and Fe2+. The enzyme activity of the former was strongly inhibited by KCN, whereas that of the latter was only slightly inhibited by 8-hydroxyquinoline.

Key words

Bacillus laterosporusFlavobacteriumgelatinprotease

Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  • A. Sharma
    • 1
  • C. L. S. N. Rao
    • 1
  • B. K. Ball
    • 1
  • S. K. Hasija
    • 1
  1. 1.Bacteriology Laboratory, Department of Biological SciencesRani Durgavati UniversityJabalpurIndia