Molecular and General Genetics MGG

, Volume 192, Issue 1, pp 110–117

Parallel induction and synthesis of PDC and ADH in anoxic maize roots

  • Andrei Laszlo
  • Patricia St. Lawrence

DOI: 10.1007/BF00327655

Cite this article as:
Laszlo, A. & St. Lawrence, P. Molec Gen Genet (1983) 192: 110. doi:10.1007/BF00327655


The activity of pyruvate decarboxylase, PDC (EC, in the primary roots of maize seedlings exposed to anoxic conditions was examined: such treatment resulted in a time-dependent increase of enzymatic activity. We identified the polypeptide associated with PDC activity by two dimensional gel electrophoresis of electrophoretic mobility variants. The incorporation of radioactive amino acids into this polypeptide under anoxic conditions indicated that the increase in enzymatic activity was accompanied by the de novo synthesis of the PDC polypeptide and therefore identified PDC as one of the anaerobic proteins of maize. The increase in PDC activity paralleled that of alcohol dehydrogenase, ADH. Since PDC and ADH catalyze sequential reactions of the Embden-Meyerhoff pathway, our results define a system of apparent coordinate regulation of the expression of two metabolically related enzymatic activities in maize.

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Andrei Laszlo
    • 1
  • Patricia St. Lawrence
    • 1
  1. 1.Genetics DepartmentUniversity of CaliforniaBerkeleyUSA
  2. 2.Department of Radiation OncologyUniversity of CaliforniaSan Francisco