Molecular and General Genetics MGG

, Volume 210, Issue 3, pp 385–393

Six chloroplast genes (ndhA-F) homologous to human mitochondrial genes encoding components of the respiratory chain NADH dehydrogenase are actively expressed: Determination of the splice sites in ndhA and ndhB pre-mRNAs

  • Tohru Matsubayashi
  • Tatsuya Wakasugi
  • Kazuo Shinozaki
  • Kazuko Yamaguchi-Shinozaki
  • Norihiro Zaita
  • Tadashi Hidaka
  • Bing Yuan Meng
  • Chikara Ohto
  • Minoru Tanaka
  • Akira Kato
  • Takeo Maruyama
  • Masahiro Sugiura
Article

DOI: 10.1007/BF00327187

Cite this article as:
Matsubayashi, T., Wakasugi, T., Shinozaki, K. et al. Mol Gen Genet (1987) 210: 385. doi:10.1007/BF00327187

Summary

Sequences (ndhA-F) homologous to human mitochondrial genes for components of the respiratory chain NADH dehydrogenase have been found in the chloroplast DnA of tobacco. The ndhA, D, E and F sequences corresponding to the mitochondrial URF1, 4, 4L and 5 are located in the small single copy region, the ndhB sequence corresponding to URF2 in the inverted repeat and the ndhC sequence corresponding to URF3 in the large single copy region of the chloroplast DNA. Northern blot hybridization revealed that all six ndh sequences are actively expressed in the chloroplasts. The ndhA and ndhB sequences contain single introns and the splice sites of their pre-mRNAs were determined by reverse transcription analysis. These findings suggest that potential components of an NADH dehydrogenase are synthesized in the chloroplasts.

Key words

ndh geneIntronNADH dehydrogenaseTranscriptionTobacco

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Tohru Matsubayashi
    • 1
  • Tatsuya Wakasugi
    • 1
  • Kazuo Shinozaki
    • 1
  • Kazuko Yamaguchi-Shinozaki
    • 1
  • Norihiro Zaita
    • 1
  • Tadashi Hidaka
    • 1
  • Bing Yuan Meng
    • 1
  • Chikara Ohto
    • 1
  • Minoru Tanaka
    • 1
  • Akira Kato
    • 2
  • Takeo Maruyama
    • 3
  • Masahiro Sugiura
    • 1
  1. 1.Center for Gene ResearchNagoya UniversityNagoyaJapan
  2. 2.National Institute of Agrobiological ResourcesTsukubaJapan
  3. 3.National Institute of GeneticsMishimaJapan