Human Genetics

, Volume 64, Issue 2, pp 110–115

Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting

  • C. R. Müller
  • K. E. Davies
  • C. Cremer
  • G. Rappold
  • J. W. Gray
  • H. H. Ropers
Original Investigations

DOI: 10.1007/BF00327104

Cite this article as:
Müller, C.R., Davies, K.E., Cremer, C. et al. Hum Genet (1983) 64: 110. doi:10.1007/BF00327104

Summary

Human Y chromosomes were purified by dual beam flow sorting from a human x Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into λgtWES.λB vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • C. R. Müller
    • 1
  • K. E. Davies
    • 2
  • C. Cremer
    • 1
  • G. Rappold
    • 1
    • 3
  • J. W. Gray
    • 4
  • H. H. Ropers
    • 1
  1. 1.Institut für Humangenetik und Anthropologie der UniversitätFreiburg i. Br.Federal Republic of Germany
  2. 2.Biochemistry DepartmentSt. Mary's Hospital Medical SchoolLondonEngland
  3. 3.Institut für Anthropologie und Humangenetik der UniversitätHeidelbergFederal Republic of Germany
  4. 4.Biomedical Science DivisionLawrence Livermore National LaboratoryLivermoreUSA