Current Genetics

, Volume 28, Issue 4, pp 384–389

High-GC primers are useful in RAPD analysis of fungi

  • Anne R. Kubelik
  • Les J. Szabo
Original Paper

DOI: 10.1007/BF00326438

Cite this article as:
Kubelik, A.R. & Szabo, L.J. Curr Genet (1995) 28: 384. doi:10.1007/BF00326438

Abstract

For genetic analysis of fungal DNAs, we have modified the RAPD method to use primers with G+C contents of 80–100%. In RAPD analysis of Puccinia graminis f. sp. tritici DNAs, these primers generated twice the number of both amplification products per primer and polymorphisms among isolates as compared to the standard 60–70% G+C primers. With respect to segregation and genetic similarity, RAPD markers generated by the high-GC primers behaved as do RAPD markers produced by the standard primers. These high-GC primers also yielded increased numbers of amplification products in RAPDs on the DNAs of a broad range of other fungi.

Key words

FungiHigh-GC primersRAPDs

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Anne R. Kubelik
    • 1
  • Les J. Szabo
    • 1
  1. 1.Cereal Rust Laboratory, Agricultural Research Service, U.S. Department of Agriculture and Department of Plant PathologyUniversity of MinnesotaSt. PaulUSA