Original Articles

Current Genetics

, Volume 26, Issue 1, pp 45-48

Electrophoretic karyotype of Mucor circinelloides

  • Á. NagyAffiliated withGedeon Richter Chemical Works
  • , Cs. VágvölgyiAffiliated withDepartment of Microbiology, Attila József University
  • , É. BallaAffiliated withGedeon Richter Chemical Works
  • , L. FerenczyAffiliated withDepartment of Microbiology, Attila József University

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Abstract

Contour-clamped homogeneous electric field (CHEF) gel electrophoresis was used to separate chromosomal size DNA molecules of two Mucor circinelloides strains. Electrophoretic karyotypes revealed the presence of eight distinct bands for the M. circinelloides f. lusitanicus strain, and four, presumably multiple, bands for the M. circinelloides f. gryseo-cyanus strain. The approximate sizes of the resolved chromosomal DNA bands ranged from 2.3 to 8.1 Mb, giving estimated genome sizes of 38.7 and 32.6 Mb, respectively. Hybridisation techniques were used to assign the leuA gene to a chromosome.

Key words

CHEF Electrophoretic karyotype Gene mapping Mucor circinelloides