Current Genetics

, Volume 26, Issue 1, pp 8–14

The yeast nuclear gene MRP-L13 codes for a protein of the large subunit of the mitochondrial ribosome


  • Lutz Grohmann
    • Institut für Genbiologische Forschung Berlin
  • Madoka Kitakawa
    • Faculty of Science, Department of BiologyUniversity of Kobe
  • Katsumi Isono
    • Faculty of Science, Department of BiologyUniversity of Kobe
  • Sabine Goldschmidt-Reisin
    • Department of BiochemistryTel-Aviv University
  • Hanns-Rüdiger Graack
    • Zentrum für Molekulare NeurobiologieUniversity Hamburg
Original Articles

DOI: 10.1007/BF00326298

Cite this article as:
Grohmann, L., Kitakawa, M., Isono, K. et al. Curr Genet (1994) 26: 8. doi:10.1007/BF00326298


The nuclear gene MRP-L13 of Saccharomyces cerevisiae, which codes for the mitochondrial ribosomal protein YmL13, has been cloned and characterized. It is a single-copy gene residing on chromosome XI. Its nucleotide sequence was found to be identical to that of the previously reported ORF YK105. A comparison of the predicted protein sequence of the MRP-L13 gene product and the actual N-terminal amino-acid sequence of the isolated YmL13 protein indicated that the mature protein is preceded by a mitochondrial signal peptide of 86 amino-acid residues, which is the longest among all known mitochondrial ribosomal proteins of S. cerevisiae. No sequence similarity was found to any other ribosomal protein in the current databases. The transcription of MRP-L13 was found to be repressed in the presence of glucose. Its protein product is not strictly essential for mitochondrial functions, but disruption of the gene by insertion of LEU2 noticeably affected cellular growth on non-fermentable carbon sources.

Key words

Saccharomyces cerevisiaeNuclear geneMitochondriaMitochondrial ribosomal protein

Copyright information

© Springer-Verlag 1994