Molecular and General Genetics MGG

, Volume 189, Issue 3, pp 471–474

Plasmidic recombination in Escherichia coliK-12: The role of recF gene function

Authors

  • Amikam Cohen
    • Department of Microbiological ChemistryThe Hebrew University, Hadassah Medical School
  • Avraham Laban
    • Department of Microbiological ChemistryThe Hebrew University, Hadassah Medical School
Article

DOI: 10.1007/BF00325911

Cite this article as:
Cohen, A. & Laban, A. Molec. Gen. Genet. (1983) 189: 471. doi:10.1007/BF00325911

Summary

Interplasmidic and intraplasmidic recombination proficiencies were determined in E. coli bacterial strains carrying rec mutations. Our results defined the role of recF gene function, recB, recC, and sbcB gene products (exonuclease V and exonuclease I) in plasmidic recombination in wild-type E. coli cells and in cells in which the recE recombination pathway is activated. RecF gene function is required for interplasmidic recombination regardless of the recB recC genotype. Intraplasmidic recombination is recF dependent in cells having a functional exonuclease V, but not in recB recC mutants. Exonuclease V activity inhibits both interplasmidic and intraplasmidic recombination via the recE pathway.

Copyright information

© Springer-Verlag 1983