Anatomy and Embryology

, Volume 177, Issue 1, pp 15–28

Ontogeny of the calcium binding protein calbindin D-28k in the rat nervous system

  • Susann Enderlin
  • A. W. Norman
  • Marco R. Celio
Original Articles

DOI: 10.1007/BF00325286

Cite this article as:
Enderlin, S., Norman, A.W. & Celio, M.R. Anat Embryol (1987) 177: 15. doi:10.1007/BF00325286

Summary

Calbindin D-28k immunoreactivity appeared at embryonal day 14 (E14) in the central nervous system as well as in the sensory organs and at E15 in the peripheral nervous system of the rat. At E14 the infundibular process of the diencephalon, cells of the posterior hypothalamus and of the dorsal thalamus were the only structures strongly immunostained in the brain, whereas neurons of the basal plate of the spinal cord, medulla oblongata and of the out-ermost layer of the cerebral cortex were only faintly labeled. Calbindin positive cerebellar Purkinje cells could be discerned at E15 together with a few cells in the hippocampus and in ganglia of the cranial nerves. At E19 various mesencephalic and metencephalic structures, spinal ganglion cells and basal ganglia displayed calbindin immunoreactive cells. The adult pattern of calbindin immunoreactivity (Garcia Segura et al. 1984) was reached before birth in most brain regions. In general, cells which displayed calbindin during brain development were also calbindin positive in the adult animal. Exceptions to this rule were cells of the deep nuclei of the cerebellum and non-neuronal cells which transiently expressed calbindin during development. Calbindin appeared in a given brain region almost invariably 1 or 2 days after the cessation of cell division and the beginning of neuronal migration and extension of neuronal processes. The calcium binding protein calbindin might influence these Ca2+-dependent processes.

Key words

OntogenyNervous systemCa2+Calcium binding proteins

Abbreviations

A

Axon

ac

anterior commissure

Acq

Aqueductus cerebri

AH

Adenohypophysis

AMY

Amygdala

aV

anterior vermis

BG

Basal Ganglia

BO

Bulbus olfactorius

BPG

basal pontine grey

C

Cortex

CA

Crista ampullaris

cer

Cerebellum

CO

otic cyst

CP

choroid plexus

CPT

Caudatoputamen

DCN

deep cerebellar nuclei

DT

dorsal thalamus

E

foregut epithelium

ec

external capsule

eml

external medullary lamina

ENP

entopeduncular nucleus

EP

Ependym

ET

Epithalamus

EY

eye

F

Fimbria

fo

Fornix

fr

Fasciculus retroflexus

GP

Globus pallidus

gr

granular laver of the cerebellum

Gsp

ganglion spirale cochlea

HI

Hippocampus

HYP

Hypothalamus

I

hippocampal interneurons

ic

internal capsule

IE

inner ear

IH

inner hair cells of the cochlea

IO

inferior olive

IS

internal sulcus cells of the cochlea

LD

laterodorsal thalamus

LV

lateral ventricle

MB

mamillary body

MH

medial habenular nucleus

mol

molecular layer of the cerebellum

mt

mamillo-thalamic tract

mtt

mamillo-tegmental tract

N

nose

n

olfactory nerve

NBM

Nucleus basalis of Meynert

NE

nose epithelium

NH

Neurohypophysis

NRT

Nucleus reticularis tegmenti pontis

ON

olfactory nuclei

OR

optic recess

PC

posterior commissure

PG

Epiphysis

PI

inferior cerebellar peduncle

PS

superior cerebellar peduncle

PU

Purkinje cell

PV

paraventricular hypothalamic nucleus

pV

posterior vermis

PVP

paraventricular thalamic nucleus

PY

pyramidal cells of the hippocampus

RA

Raphe nuclei

RE

Nucleus reuniens

RH

Nucleus rhomboideus

RN

reticular nucleus

RP

Rathke's pouch

sc

spinal cord

SG

substantia gelatinosa Rolandi

slm

Stratum-lacunosum molecular

sm

Stria medullaris

SN

Substantia nigra

SO

supraoptic nucleus

Sol

Nucleus of the solitary tract

Tect

Tectum

Teg

Tegmentum

TG

tegmental nucleus of Gudden

Tg

tongue

TO

tuberculum olfactorium

VT

ventral thalamus

WH

white matter

ZI

Zona incerta

3

third ventricle

4

fourth ventricle

IIN

Nervus opticus

Vg

Trigeminal ganglion

Vsp

spinal trigeminal nucleus

VIIG

Ganglion geniculi

VIIIG

Ganglion vestibulare

IXGI

interior ganglion of IX

IXGS

superior ganglion of IX

XGI

inferior ganglion of X

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Susann Enderlin
    • 1
  • A. W. Norman
    • 2
  • Marco R. Celio
    • 1
  1. 1.Anatomisches Institut der Universität ZürichZürichSwitzerland
  2. 2.Biochemistry DepartmentUniversity of California RiversideRiversideUSA
  3. 3.Anatomisches Institut der Universität KielKielGermany