Molecular and General Genetics MGG

, Volume 212, Issue 1, pp 129-133

First online:

A transposon-like structure in the 5′ flanking sequence of a legumin gene from Pisum sativum

  • A. H. ShirsatAffiliated withDepartment of Botany, Durham University, Science Laboratories

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The legumin storage proteins of Pisum sativum are coded for by a multigene family. An insertion element (Pis1) has been found integrated into the 5′ flanking sequence of the legC legumin seed storage protein gene. This element contains all the sequence features of the CACTA family of insertion elements, has perfect 12 bp inverted repeats at its termini, and generates a target host site duplication upon integration. An 8 bp sequence within the left arm of the insertion element shows perfect homology to a sequence in the legC flanking region. Three stem-loop structures which can be formed within the element have the same stem sequence.

Key words

Pea legumin gene Transposon