Fresenius' Journal of Analytical Chemistry

, Volume 343, Issue 6, pp 523–525

Caffeine and theobromine in coffee, tea and cola-beverages

Simultaneous determination by reversed-phase ion-interaction HPLC
  • M. C. Gennaro
  • C. Abrigo
Original Papers Food And Pharmaceutical Products

DOI: 10.1007/BF00322162

Cite this article as:
Gennaro, M.C. & Abrigo, C. Fresenius J Anal Chem (1992) 343: 523. doi:10.1007/BF00322162


A new method based on reversed-phase ion-interaction HPLC is described for the identification and quantitation of theobromine, theophylline and caffeine in beverages. The interaction reagent used is octylamine orto-phosphate which also constitutes the mobile phase. The stationary phase is a microparticulate reversed-phase C-18 packing. With spectrophotometric detection at 274 nm, detection levels of 0.15, 0.30 and 0.40 ppm were achieved for theobromine, theophylline and caffeine, respectively.

Quantitative analysis was performed by the standard addition method for theobromine and caffeine contents in tea, espresso-coffee, decaffeinated coffee, decaffeinated tea and cola-beverages.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • M. C. Gennaro
    • 1
  • C. Abrigo
    • 1
  1. 1.Dipartimento di Chimica AnaliticaUniversitá di TorinoTorinoItaly