Current Genetics

, Volume 21, Issue 1, pp 83–84

One-step transformation of yeast in stationary phase

  • Dz-Chi Chen
  • Bei-Chang Yang
  • Tsong-Teh Kuo
Short Communications

DOI: 10.1007/BF00318659

Cite this article as:
Chen, DC., Yang, BC. & Kuo, TT. Curr Genet (1992) 21: 83. doi:10.1007/BF00318659

Summary

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

Key words

YeastTransformationThio compoundStationary phase

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Dz-Chi Chen
    • 1
    • 3
  • Bei-Chang Yang
    • 3
  • Tsong-Teh Kuo
    • 1
    • 2
    • 3
  1. 1.Graduate Institute of BotanyNational Taiwan UniversityTaipeiRepublic of China
  2. 2.Institute of BotanyAcademia SinicaTaipeiRepublic of China
  3. 3.Institute of Molecular BiologyAcademia SinicaTaipeiRepublic of China