Current Genetics

, Volume 21, Issue 1, pp 83–84

One-step transformation of yeast in stationary phase

Authors

  • Dz-Chi Chen
    • Graduate Institute of BotanyNational Taiwan University
    • Institute of Molecular BiologyAcademia Sinica
  • Bei-Chang Yang
    • Institute of Molecular BiologyAcademia Sinica
  • Tsong-Teh Kuo
    • Graduate Institute of BotanyNational Taiwan University
    • Institute of BotanyAcademia Sinica
    • Institute of Molecular BiologyAcademia Sinica
Short Communications

DOI: 10.1007/BF00318659

Cite this article as:
Chen, D., Yang, B. & Kuo, T. Curr Genet (1992) 21: 83. doi:10.1007/BF00318659

Summary

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45°C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5×108 cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 104 transformants/μg plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

Key words

YeastTransformationThio compoundStationary phase
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Copyright information

© Springer-Verlag 1992