Acta Neuropathologica

, Volume 90, Issue 6, pp 626–632

Monoamine oxidase-B-positive granular structures in the hippocampus of aged senescence-accelerated mouse (SAMP8)

  • Shinichi Nakamura
  • Ichiro Akiguchi
  • Naoyuki Seriu
  • Kazunori Ohnishi
  • Manabu Takemura
  • Masaki Ueno
  • Hidekazu Tomimoto
  • Toshio Kawamata
  • Jun Kimura
  • Masanori Hosokawa
Regular Paper

DOI: 10.1007/BF00318576

Cite this article as:
Nakamura, S., Akiguchi, I., Seriu, N. et al. Acta Neuropathol (1995) 90: 626. doi:10.1007/BF00318576

Abstract

We examined the histochemical localization of monoamine oxidase in the hippocampus of young and old senescence-accelerated mouse (SAM). We found a monoamine oxidase-B-positive granular structure (MGS) in the hippocampus of old SAMP8, an accelerated senescenceprone line of SAM. The MGS was a round-shaped granular structure of 0.5 to 5 μm diameter and usually formed a cluster, the largest diameter of which ranged from 50 to 150 μm. No MGS were found in the hippocampus of young SAMP8 or of young SAMR1, an accelerated senescence resistant line of SAM, and only few, if any, were seen in old SAMR1. A monoamine oxidase-positive astrocyte was usually observed in the central area of each cluster of MGS. Furthermore, the MGS was in close anatomical relationship with monoamine oxidase-positive astrocytic processes. The enzyme inhibition experiments showed that monoamine oxidase activities localized in the MGS and astrocytes were both predominantly of type B. These findings suggest MGS occurs at least partly in monoamine oxidase-B-positive astrocytes. Furthermore, the MGS was similar to a periodic acid-Schiff-positive granular structure, a polyglucosan body previously documented in the brains of old SAMP8 and some other aged mice strains including C57BL/6 and nude mice, in terms of their size, morphological appearances and topographical distribution in the hippocampus. Thus, the present results suggest that monoamine oxidase type B is a proteinaceous component of the periodic acid-Schiff-positive granular structure in aged mice brains, and might provide some clues for clarifying the mechanisms of age-related occurrence of periodic acid-Schiff-positive granular structures in mice brains.

Key words

Monoamine oxidase-BSenescence-accelerated mouse (SAM)Monoamine oxidase-B-positive granular structurePeriodic acid-Schiff-positive granular structureAging

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Shinichi Nakamura
    • 1
  • Ichiro Akiguchi
    • 1
  • Naoyuki Seriu
    • 1
  • Kazunori Ohnishi
    • 1
  • Manabu Takemura
    • 1
  • Masaki Ueno
    • 1
  • Hidekazu Tomimoto
    • 1
  • Toshio Kawamata
    • 1
  • Jun Kimura
    • 1
  • Masanori Hosokawa
    • 2
  1. 1.Department of Neurology, Faculty of MedicineKyoto UniversityKyotoJapan
  2. 2.Department of Senescence Biology Chest Disease Research InstituteKyoto UniversityKyotoJapan