Current Genetics

, Volume 22, Issue 4, pp 277–282

The REV2 gene of Saccharomyces cerevisiae: cloning and DNA sequence

Authors

  • F. Ahne
    • GSF-Forschungszentrum für Umwelt und GesundheitInstitut für Strahlenbiologie
  • M. Baur
    • GSF-Forschungszentrum für Umwelt und GesundheitInstitut für Strahlenbiologie
  • F. Eckardt-Schupp
    • GSF-Forschungszentrum für Umwelt und GesundheitInstitut für Strahlenbiologie
Original Articles

DOI: 10.1007/BF00317921

Cite this article as:
Ahne, F., Baur, M. & Eckardt-Schupp, F. Curr Genet (1992) 22: 277. doi:10.1007/BF00317921

Summary

The REV2 gene of Saccharomyces cerevisiae was cloned and sequenced; it contains an open reading frame of 1985 bp with a coding potential of 662 amino acids. Interruption of the chromosomal REV2 gene by integrating the URA3 gene coupled with partial deletion of the 3′ terminal region produced viable haploid rev2Δ mutants. This indicates that the REV2 gene is non-essential for growth. The rev2Δ mutant is slightly more UV-sensitive than strains carrying various rev2 alleles (rev2-1, rev2x, rad5-1, rad5-8). The putative Rev2 protein is probably a globular protein containing a highly conserved nucleotide-binding site and two zinc-finger domains.

Key words

Yeast DNA-repair Mutation-deficient mutant Nucleotide-binding consensus

Copyright information

© Springer-Verlag 1992