Archives of Toxicology

, Volume 55, Issue 1, pp 11–15

The effectiveness of N-acetylcysteine in isolated hepatocytes, against the toxicity of paracetamol, acrolein, and paraquat

  • Janet R. Dawson
  • Kajsa Norbeck
  • Irene Anundi
  • Peter Moldéus
Original Investigations

DOI: 10.1007/BF00316579

Cite this article as:
Dawson, J.R., Norbeck, K., Anundi, I. et al. Arch Toxicol (1984) 55: 11. doi:10.1007/BF00316579

Abstract

The protective effect of N-acetylcysteine against the toxicity of paracetamol, acrolein, and paraquat was investigated using isolated hepatocytes as the experimental system. N-acetylcysteine protects against paracetamol toxicity by acting as a precursor for intracellular glutathione. N-acetylcysteine protects against acrolein toxicity by providing a source of sulfhydryl groups, and is effective without prior conversion. Paraquat toxicity can be decreased by coincubating the cells with N-acetylcysteine, but the mechanism for the protective effect is not as clear in this instance. It is probable that N-acetylcysteine protects against paraquat toxicity by helping to maintain intracellular glutathinone levels.

Key words

HepatocytesN-AcetylcysteineParacetamolAcroleinParaquatGlutathione

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Janet R. Dawson
    • 1
  • Kajsa Norbeck
    • 1
  • Irene Anundi
    • 1
  • Peter Moldéus
    • 1
  1. 1.Department of Forensic MedicineKarolinska InstitutetStockholmSweden