Current Genetics

, Volume 27, Issue 6, pp 493–495

Gene PSO5 of Saccharomyces cerevisiae, involved in repair of oxidative DNA damage, is allelic to RAD16

  • S. O. Paesi-Toresan
  • C. T. Pich
  • M. Grey
  • D. Keszenman-Pereyra
  • M. Brendel
  • J. A. P. Henriques
Original Paper

DOI: 10.1007/BF00314437

Cite this article as:
Paesi-Toresan, S.O., Pich, C.T., Grey, M. et al. Curr Genet (1995) 27: 493. doi:10.1007/BF00314437

Abstract

The pos5-1 mutation renders Saccharomyces cerevisiae cells sensitive to DNA-damaging agents. We have isolated plasmids from a S. cerevisiae genomic library capable of restoring wild-type levels of 254-nm ultraviolet light sensitivity of the pso5-1 mutant. DNA sequence analysis revealed that the complementing activity resides in RAD16, a gene involved in excision repair. Tetrad analysis showed that PSO5, like RAD16, is tightly linked to LYS2 on chromosome II. Moreover, allelism between the pso5-1 and rad16 mutants was demonstrated by the comparison of mutagen sensitivity phenotypes, complementation tests, and by meiotic analysis. The cloned RAD16 gene was capable of restoring wild-type resistance of the pso5-1 mutant to H2O2 and photoactivated 3-carbethoxypsoralen, both treatments generating oxidative stress-related DNA damage. This indicates that RAD16/PSO5 might also participate in the repair of oxidative base damage.

Key words

DNA Repair Psoralen photoaddition Yeast mutant Oxidative DNA damage 
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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • S. O. Paesi-Toresan
    • 1
  • C. T. Pich
    • 1
  • M. Grey
    • 2
  • D. Keszenman-Pereyra
    • 1
  • M. Brendel
    • 2
  • J. A. P. Henriques
    • 1
  1. 1.Departmento de Biofísica — Centro de BiotecnologiaUniversidade Federal do Rio Grando do SulPorto AlegreBrasil
  2. 2.Institut für MikrobiologieJ. W.-Goethe-UniversitätFrankfurt/MainGermany

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