Current Genetics

, Volume 26, Issue 2, pp 95–99

Mistranslation of human phosphoglycerate kinase in yeast in the presence of paromomycin

  • Chris M. Grant
  • Mick F. Tuite
Original Articles

DOI: 10.1007/BF00313794

Cite this article as:
Grant, C.M. & Tuite, M.F. Curr Genet (1994) 26: 95. doi:10.1007/BF00313794

Abstract

Missense errors in the translation of mRNAs in Saccharomyces cerevisiae were screened by looking for charge heterogeneity of proteins on two-dimensional gels resulting from the substitution of charged and neutral amino acids. No such mistranslation was detected in wild-type yeast strains grown in the presence of the translational error-inducing antibiotic paromomycin. However, paromomycin-induced mistranslation of a heterologous mRNA, encoding human phosphoglycerate kinase expressed in yeast, was seen. We suggest that the combination of error-prone translation of a heterologous mRNA, and growth in the presence of paromomycin, leads to an accumulation of mistranslated proteins that can be detected by two-dimensional gel electrophoresis.

Key words

Translational fidelityParomomycinStutteringSaccharomyces cerevisiae

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Chris M. Grant
    • 1
  • Mick F. Tuite
    • 1
  1. 1.Biological LaboratoryUniversity of KentCanterburyUK
  2. 2.Section on Molecular Genetics of Lower Eukaryotes, Laboratory of Molecular Genetics, National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaUSA