Current Genetics

, Volume 27, Issue 2, pp 102–109

Interactions among mutations affecting spontaneous mutation, mitotic recombination, and DNA repair in yeast

  • Beth A. Montelone
  • Katherine J. Koelliker
Original Paper

DOI: 10.1007/BF00313423

Cite this article as:
Montelone, B.A. & Koelliker, K.J. Curr Genet (1995) 27: 102. doi:10.1007/BF00313423

Abstract

The mutant alleles mms9-1, mms13-1, or mms21-1 of Saccharomyces cerevisiae confer pleiotropic effects, including sensitivity to the alkylating agent methyl methanesulfonate, elevations in spontaneous mutation and mitotic recombination, defects in meiosis, and cross-sensitivity to radiation. We constructed double-mutant strains containing an mms mutation and a defect in either excision repair, mutagenic repair, or recombinational repair and measured the levels of spontaneous mutation and mitotic reombination. Double mutants lacking excision repair show elevations in spontaneous mutation but with predominantly unchanged levels of mitotic recombination. RAD52 function was required for the expression of the hyper-recombination phenotype of the mms9-1, mms13-1, and mms21-1 alleles; double mutants displayed the very low recombination levels characteristic of rad52 mutants. Phenotypes of double mutants containing one of the mms alleles and either of the hyper-recombination/mutator rad6-1 or rad3-102 alleles suggest that the mutagenic lesions in mms strains may not be identical to the recombinogenic lesions.

Key words

Hyper-recombination mutantsMutators Nucleotide excision repair

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Beth A. Montelone
    • 1
  • Katherine J. Koelliker
    • 1
  1. 1.Division of BiologyKansas State UniversityManhattanUSA