Original Articles

Current Genetics

, Volume 17, Issue 1, pp 13-19

Construction of an h +Sstrain of Schizosaccharomyces pombe

  • Lutz HeimAffiliated withInstitut für Genetik, Technische Universität Braunschweig

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By spontaneous in vivo integration of a mat2:1° plasmid, containing a Plus (P) cassette, into an h -LMT region of Schizosaccharomyces pombe an h + strain was obtained which neither mutates to h - nor to h 90. Southern blotting showed that it possesses the same mating-type (MT) configuration as h -S except that P information resides in both cassettes. Therefore the strain was called h +S. By crossing h +S with the h - strain LK42 of Engelke et al. (1987) it was possible to obtain h - recombinants with the MT configuration mat1:1(M)smt-o-L-mat2:3(P). Because of the totally defective smt signal (smt-o) in these recombinants no MT switching occurs, so that M information is conserved in mat1:1; furthermore the cassette mat2:3(P) is not expressed like in strains with a K region. This proves that the K region does not cause the silencing of mat2:3(P).

Key words

Mating type h +Sstrain K region Schizosaccharomyces pombe