Current Genetics

, Volume 23, Issue 5, pp 463–467

Genomic heterogeneity in the yeast Candida parapsilosis

Authors

  • Timothy J. Lott
    • Division of Bacterial and Mycotic DiseasesNational Center for Infectious Diseases, Center for Disease Control
  • Randall J. Kuykendall
    • Division of Bacterial and Mycotic DiseasesNational Center for Infectious Diseases, Center for Disease Control
  • Sharon F. Welbel
    • Hospital Infections ProgramNational Center for Infectious Diseases, Centers for Disease Control
  • Arun Pramanik
    • Department of NeonatologyLouisiana State University Medical Center
  • Brent A. Lasker
    • Division of Bacterial and Mycotic DiseasesNational Center for Infectious Diseases, Center for Disease Control
Original Articles

DOI: 10.1007/BF00312635

Cite this article as:
Lott, T.J., Kuykendall, R.J., Welbel, S.F. et al. Curr Genet (1993) 23: 463. doi:10.1007/BF00312635

Abstract

Candida parapsilosis shows a wide intraspecies variation in chromosome/homolog size distribution. As a prerequisite for delineating modes of transmission, we have undertaken an analysis of genetic variation at different levels. In the present study we have observed that a majority of isolates display similar electrophoretic karyotype patterns consistent for the species, with variations in the smaller group of chromosomes. In two strains we observed phenotypic “switching”; one of these also exhibited a mixed karyotypic subpopulation. In contrast, a few isolates displayed a greater degree of chromosome/ homolog size variation. We also observed, through randomly amplified polymorphic DNA (RAPD) analysis, results consistent with those of pulsed-field electrophoresis. Isolates displaying a high degree of chromosome/homolog variation also displayed a high degree of variation in genomic “fingerprints”. Polymorphisms, although present, were much reduced in the majority of isolates. These parallel observations suggest a common underlying mechanism. Our results are consistent with the hypothesis that chromosome-sized variations in C. parapsilosis are due to random genetic events. A similar mechanism has been hypothesized for the taxonomically related yeast Candida albicans.

Key words

Candida parapsilosisPulsed-field electrophoresisElectrophoretic karyotypePolymerase chain reactionGenomic fingerprint

Copyright information

© Springer-Verlag 1993