Current Genetics

, Volume 23, Issue 5, pp 463–467

Genomic heterogeneity in the yeast Candida parapsilosis

  • Timothy J. Lott
  • Randall J. Kuykendall
  • Sharon F. Welbel
  • Arun Pramanik
  • Brent A. Lasker
Original Articles

DOI: 10.1007/BF00312635

Cite this article as:
Lott, T.J., Kuykendall, R.J., Welbel, S.F. et al. Curr Genet (1993) 23: 463. doi:10.1007/BF00312635

Abstract

Candida parapsilosis shows a wide intraspecies variation in chromosome/homolog size distribution. As a prerequisite for delineating modes of transmission, we have undertaken an analysis of genetic variation at different levels. In the present study we have observed that a majority of isolates display similar electrophoretic karyotype patterns consistent for the species, with variations in the smaller group of chromosomes. In two strains we observed phenotypic “switching”; one of these also exhibited a mixed karyotypic subpopulation. In contrast, a few isolates displayed a greater degree of chromosome/ homolog size variation. We also observed, through randomly amplified polymorphic DNA (RAPD) analysis, results consistent with those of pulsed-field electrophoresis. Isolates displaying a high degree of chromosome/homolog variation also displayed a high degree of variation in genomic “fingerprints”. Polymorphisms, although present, were much reduced in the majority of isolates. These parallel observations suggest a common underlying mechanism. Our results are consistent with the hypothesis that chromosome-sized variations in C. parapsilosis are due to random genetic events. A similar mechanism has been hypothesized for the taxonomically related yeast Candida albicans.

Key words

Candida parapsilosisPulsed-field electrophoresisElectrophoretic karyotypePolymerase chain reactionGenomic fingerprint

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Timothy J. Lott
    • 1
  • Randall J. Kuykendall
    • 1
  • Sharon F. Welbel
    • 2
  • Arun Pramanik
    • 3
  • Brent A. Lasker
    • 1
  1. 1.Division of Bacterial and Mycotic DiseasesNational Center for Infectious Diseases, Center for Disease ControlAtlantaUSA
  2. 2.Hospital Infections ProgramNational Center for Infectious Diseases, Centers for Disease ControlAtlantaUSA
  3. 3.Department of NeonatologyLouisiana State University Medical CenterShreveportUSA
  4. 4.Centers for Disease ControlAtlantaUSA