Current Genetics

, Volume 23, Issue 5, pp 392–396

Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae

Authors

  • Michael J. Dorsey
    • Department of Biological Sciences, ML 006University of Cincinnati
  • Paula Hoeh
    • Department of Biological Sciences, ML 006University of Cincinnati
  • Charlotte E. Paquin
    • Department of Biological Sciences, ML 006University of Cincinnati
Original Articles

DOI: 10.1007/BF00312624

Cite this article as:
Dorsey, M.J., Hoeh, P. & Paquin, C.E. Curr Genet (1993) 23: 392. doi:10.1007/BF00312624

Abstract

Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.

Key words

Saccharomyces cerevisiaeGene amplificationADH4CUP1

Copyright information

© Springer-Verlag 1993