Original Articles

Current Genetics

, Volume 23, Issue 5, pp 392-396

First online:

Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae

  • Michael J. DorseyAffiliated withDepartment of Biological Sciences, ML 006, University of Cincinnati
  • , Paula HoehAffiliated withDepartment of Biological Sciences, ML 006, University of Cincinnati
  • , Charlotte E. PaquinAffiliated withDepartment of Biological Sciences, ML 006, University of Cincinnati

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Abstract

Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.

Key words

Saccharomyces cerevisiae Gene amplification ADH4 CUP1