Phenotypic identification of amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae
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- Dorsey, M.J., Hoeh, P. & Paquin, C.E. Curr Genet (1993) 23: 392. doi:10.1007/BF00312624
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Primary gene amplification, i.e., mutation from one gene copy to multiple gene copies per genome, is important in genomic evolution, as a means of producing anti-cancer drug resistance, and is associated with the progression of tumor malignancy. Primary amplification has not been studied in normal eukaryotic cells because amplifications are extremely rare in these cells. A system has been developed to phenotypically identify co-amplifications of the ADH4 and CUP1 genes of Saccharomyces cerevisiae and 21 independent spontaneous amplifications have been isolated.