Current Genetics

, Volume 18, Issue 2, pp 161–166

Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): Indications of a pectin lyase gene family in A. niger

Authors

  • J. A. M. Harmsen
    • Department of Genetics, Section Molecular GeneticsAgricultural University
  • M. A. Kusters-van Someren
    • Department of Genetics, Section Molecular GeneticsAgricultural University
  • J. Visser
    • Department of Genetics, Section Molecular GeneticsAgricultural University
Original Articles

DOI: 10.1007/BF00312604

Cite this article as:
Harmsen, J.A.M., Kusters-van Someren, M.A. & Visser, J. Curr Genet (1990) 18: 161. doi:10.1007/BF00312604

Summary

Using the previously cloned Aspergillus niger N756 pectin lyase D gene as a probe, the corresponding pelD gene has been isolated from a genomic library of the loboratory strain A. niger N400. This gene encodes PLD, previously described as PLI, which is one of the two major pectin lyases isolated from the commeriial pectinase preparation Ultrazym®. Heterologous hybridization of the A. niger N400 genomic library with the pelD gene led to the isolation of another five genes: pelA, B, C, E, and F. These genes differ in their hybridization patterns with probes containing either the entire pelD gene, or 5′ or 3′ parts thereof. By partial sequencing, and expression in an A. niger transformant containing multiple copies of the pelA gene, we show that this gene, which hybridizes strongest with the pelD gene, encodes the other major pectin lyase from Ultrazym®, PLII.

Key words

Aspergillus nigerpel genesPectin lyaseHeterologous hybridization

Copyright information

© Springer-Verlag 1990