Current Genetics

, Volume 28, Issue 1, pp 12–18

Mating-type suppression of the DNA-repair defect of the yeast rad6Δ mutation requires the activity of genes in the RAD52 epistasis group

  • Yu-Xin Yan
  • Louise Prakash
  • Robert H. Schiestl
Original Paper

DOI: 10.1007/BF00311876

Cite this article as:
Yan, YX., Prakash, L. & Schiestl, R.H. Curr Genet (1995) 28: 12. doi:10.1007/BF00311876


The RAD6 gene of Saccharomyces cerevisiae is required for post-replication repair of UV-damaged DNA, UV mutagenesis, and sporulation. Here, we show that the radiation sensitivity of a MATarad6Δ strain can be suppressed by the MATα2 gene carried on a multicopy plasmid. The a1-α2 suppression is specific to the RAD6 pathway, as mutations in genes required for nucleotide excision repair or for recombinational repair do not show such mating-type suppression. The a1-α2 suppression of the rad6Δ mutation requires the activity of the RAD52 group of genes, suggesting that suppression occurs by channelling of post-replication gaps present in the rad6Δ mutant into the RAD52 recombinational repair pathway. The a1-α2 repressor could mediate this suppression via an enhancement in the expression, or the activity, of recombination genes.

Key words

DNA repair RAD6 gene RAD52 gene UV mutagenesis Mating type 

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Yu-Xin Yan
    • 1
  • Louise Prakash
    • 1
  • Robert H. Schiestl
    • 2
  1. 1.Department of BiophysicsUniversity of Rochester School of MedicineRochesterUSA
  2. 2.Department of BiologyUniversity of RochesterRochesterUSA
  3. 3.Gastrointestinal Unit, Jackson 904, Massachusetts General HospitalHarvard Medical SchoolBostonUSA
  4. 4.Department of Molecular and Cellular ToxicologyHarvard School of Public HealthBostonUSA
  5. 5.Sealy Center for Molecular ScienceUTMBGalvestonUSA

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