Current Genetics

, Volume 27, Issue 5, pp 417–426

A mutant allele of the SUP45 (SAL4) gene of Saccharomyces cerevisiae shows temperature-dependent allosuppressor and omnipotent suppressor phenotypes

Authors

  • I. Stansfield
    • Research School of BiosciencesUniversity of Kent
  • Akhmaloka
    • Research School of BiosciencesUniversity of Kent
  • M. F. Tuite
    • Research School of BiosciencesUniversity of Kent
Original Paper

DOI: 10.1007/BF00311210

Cite this article as:
Stansfield, I., Akhmaloka & Tuite, M.F. Curr Genet (1995) 27: 417. doi:10.1007/BF00311210
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Abstract

Using a plasmid-based termination-read-through assay, the sal4-2 conditional-lethal (temperature-sensitive) allele of the SUP45 (SAL4) gene was shown to enhance the efficiency of the weak ochre suppressor tRNA SUQ5 some 10-fold at 30°C. Additionally, this allele increased the suppressor efficiency of SRM2-2, a weak tRNAGln ochre suppressor, indicating that the allosuppressor phenotype is not SUQ5-specific. A sup+ sal4-2 strain also showed a temperature-dependent omnipotent suppressor phenotype, enhancing readthrough of all three termination codons. Combining the sal4-2 allele with an efficient tRNA nonsense suppressor (SUP4) increased the temperature-sensitivity of that strain, indicating that enhanced nonsense suppressor levels contribute to the conditional-lethality conferred by the sal4-2 allele. However, UGA suppression levels in a sup+ sal4-2 strain following a shift to the non-permissive temperature reached a maximum significantly below that exhibited by a non-temperature sensitive SUP4 suppressor strain. Enhanced nonsense suppression may not therefore be the primary cause of the conditional-lethality of this allele. These data indicate a role for Sup45p in translation termination, and possibly in an additional, as yet unidentified, cellular process.

Key words

Saccharomyces cerevisiaeOmnipotent suppressionNonsense suppressionSUP45
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© Springer-Verlag 1995