Histochemie

, Volume 36, Issue 1, pp 1–4

Carbonic anhydrase activity in Rana pipiens skin: biochemical and histochemical analysis

Authors

  • Seymour Rosen
    • Department of PathologyBeth Israel Hospital and Harvard Medical School
  • Nancy Jane Friedley
    • Department of PathologyBeth Israel Hospital and Harvard Medical School
Article

DOI: 10.1007/BF00310114

Cite this article as:
Rosen, S. & Friedley, N.J. Histochemie (1973) 36: 1. doi:10.1007/BF00310114
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Summary

Carbonic anhydrase activity was demonstrated biochemically and localized histochemically in epidermis of Rana pipiens. The activity observed throughout the lower epidermis was diminished or abolished by periods of fixation which did not affect the activity in upper epidermis. In the latter region, the enzyme was demonstrated only in certain cells with a flask shape known to have numerous mitochondria and a system of convoluted apical membranes. Such findings raise the possibility that these distinctive cells play a role in transport functions of the isolated frog skin suppressed by carbonic anhydrase inhibitors.

Copyright information

© Springer-Verlag 1973