Cell and Tissue Research

, Volume 262, Issue 2, pp 339–349

Brush cells of the mouse gallbladder

A correlative light- and electron-microscopical study
  • L. Luciano
  • E. Reale

DOI: 10.1007/BF00309889

Cite this article as:
Luciano, L. & Reale, E. Cell Tissue Res (1990) 262: 339. doi:10.1007/BF00309889


The brush cells (BC) of the mouse gallbladder were studied using light and electron microscopy (transmission and scanning) to determine their shape and distribution. Specimens were fixed in glutaraldehyde and postfixed in ferrocyanide-reduced osmium tetroxide. BC selectively stained with toluidine blue could be identified by means of light microscopy and subsequently studied in serial semithin and ultrathin sections. The results revealed that the shape of the BC is flask-like. A slender, occasionally branched cytoplasmic process emerges from the bulk cell body and extends through the basal region of neighboring epithelial elements to the basement membrane. Examination of the entire gallbladder epithelial surface by scanning electron microscopy revealed that the BC are numerous in the neck region of the organ but only scanty or even absent in wide areas of the corpus region. Their number increases again in the fundic region. These results demonstrate a preferential regional distribution of BC in the gallbladder, which is discussed in relation to a possible functional significance of the BC.

Key words

Brush cellsGallbladderMicrovilliLight- and electron microscopyMouse (NMRI)

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • L. Luciano
    • 1
  • E. Reale
    • 1
  1. 1.Laboratory of Cell Biology and Electron MicroscopyHannover Medical SchoolHannoverFederal Republic of Germany
  2. 2.Abteilung Zellbiologie und ElektronenmikroskopieMedizinische Hochschule HannoverHannover 61Federal Republic of Germany