Current Genetics

, Volume 26, Issue 3, pp 251–255

Transformation of Botrytis cinerea with the hygromycin B resistance gene, hph

  • W. Hamada
  • Ph. Reignault
  • G. Bompeix
  • M. Boccara
Original Articles

DOI: 10.1007/BF00309556

Cite this article as:
Hamada, W., Reignault, P., Bompeix, G. et al. Curr Genet (1994) 26: 251. doi:10.1007/BF00309556

Abstract

A transformation method has been developed for the phytopathogenic fungus Botrytis cinerea. Protoplasts were transformed with pAN7-1 plasmid carrying the Escherichia coli hygromycin phosphotransferase gene (hph), confering hygromycin B resistance, downstream from an Aspergillus nidulans promoter. Molecular analysis showed that transformation resulted in an integration of the plasmid into different regions of the B. cinerea genome and occurred through non-homologous recombination. The frequency was 2–10 transformants per μg of DNA. Transformants expressed phosphotransferase activity confirming that the hph gene conferred the hygromycin-resistance phenotype. All transformants analysed so far proved to be stable after several subcultures without any selective pressure.

Key words

Botrytis cinerea Hygromycin resistance Non-homologous recombination Phytopathogenic fungus 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • W. Hamada
    • 1
  • Ph. Reignault
    • 1
  • G. Bompeix
    • 1
  • M. Boccara
    • 1
    • 2
  1. 1.Laboratoire de biochimie et de pathologie végétalesUPMCParis cedex 05France
  2. 2.Laboratoire de pathologie végétaleINA ParisParis cedex 05France