Histochemie

, Volume 34, Issue 3, pp 191–203

Demonstration of the neural crest origin of type I (APUD) cells in the avian carotid body, using a cytochemical marker system

Authors

  • A. G. E. Pearse
    • Department of HistochemistryRoyal Postgraduate Medical School, Hammersmith Hospital
    • Laboratoire d'Embryologie de la Faculté des Sciences de Nantes
  • J. M. Polak
    • Department of HistochemistryRoyal Postgraduate Medical School, Hammersmith Hospital
    • Laboratoire d'Embryologie de la Faculté des Sciences de Nantes
  • F. W. D. Rost
    • Department of HistochemistryRoyal Postgraduate Medical School, Hammersmith Hospital
    • Laboratoire d'Embryologie de la Faculté des Sciences de Nantes
  • J. Fontaine
    • Department of HistochemistryRoyal Postgraduate Medical School, Hammersmith Hospital
    • Laboratoire d'Embryologie de la Faculté des Sciences de Nantes
  • C. Le Lièvre
    • Department of HistochemistryRoyal Postgraduate Medical School, Hammersmith Hospital
    • Laboratoire d'Embryologie de la Faculté des Sciences de Nantes
  • N. Le Douarin
    • Department of HistochemistryRoyal Postgraduate Medical School, Hammersmith Hospital
    • Laboratoire d'Embryologie de la Faculté des Sciences de Nantes
Article

DOI: 10.1007/BF00303435

Cite this article as:
Pearse, A.G.E., Polak, J.M., Rost, F.W.D. et al. Histochemie (1973) 34: 191. doi:10.1007/BF00303435

Summary

The biogenic amines present in the carotid body Type 1 cells of two avian species (Japanese quail and chicken) were identified, by microspectrofluorometry of formaldehyde-induced fluorescence, as dopamine and 5-hydroxytryptamine respectively. These and other cytochemical properties establish the cells as members of the APUD series.

Grafts of the neural rhombencephalic primordium from 6 to 10-somite quail embryos were implanted in the appropriate region of chick embryos of the same age. After up to 11 days incubation the carotid bodies of the host were freeze-dried and treated with hot formaldehyde vapour. The carotid body Type 1 cells in the chick host were identified, by the presence of dopamine and the absence of 5-HT, as cells from the quail neural crest.

The dopamine phenotype in cells of quail origin thus provides a cytochemical marker which may be used for other allograft experiments. The present work confirms earlier findings, using a biological (nuclear chromatin) marker, which showed the avian carotid body to be of neural crest origin.

Copyright information

© Springer-Verlag 1973