Archives of Toxicology

, Volume 63, Issue 5, pp 351–355

Metabolism of methyl chloride by human erythrocytes

Authors

  • H. Peter
    • Institut für Arbeitsphysiologie an der Universität Dortmund
  • S. Deutschmann
    • Institut für Arbeitsphysiologie an der Universität Dortmund
  • C. Reichel
    • Institut für Arbeitsphysiologie an der Universität Dortmund
  • E. Hallier
    • Institut für Arbeitsphysiologie an der Universität Dortmund
Original Articles

DOI: 10.1007/BF00303122

Cite this article as:
Peter, H., Deutschmann, S., Reichel, C. et al. Arch Toxicol (1989) 63: 351. doi:10.1007/BF00303122

Abstract

Erythrocyte cytoplasm of rats, mice and humans was incubated in head space vials with methyl chloride and the decline in concentration of the substance monitored as a parameter of metabolism. The production of S-methylglutathione was controlled by tlc. Rats, mice, bovines, pigs, sheep and rhesus monkeys showed no conversion of methyl chloride in erythrocyte cytoplasm. About 60% of the human blood samples showed a significant metabolic elimination of the substance (conjugators), whereas about 40% did not (non-conjugators). The production of S-methylglutathione indicated enzymatic metabolism of the substance by glutathione S-transferases. In literature, a “major” and “minor” form of human erythrocyte glutathione S-transferase has been described. The results indicate that the “minor” form is probably responsible for the unique metabolism of methyl chloride in human erythrocytes.

Key words

ErythrocytesHumanMethyl chlorideGlutathione S-transferases

Copyright information

© Springer-Verlag 1989