Journal of Comparative Physiology B

, Volume 165, Issue 8, pp 622–627

The stress-70 protein family in diplopods: induction and characterization

  • M. Zanger
  • G. Alberti
  • M. Kuhn
  • H. -R. Köhler
Original Paper

DOI: 10.1007/BF00301130

Cite this article as:
Zanger, M., Alberti, G., Kuhn, M. et al. J Comp Physiol B (1996) 165: 622. doi:10.1007/BF00301130

Abstract

To induce stress-70 proteins (hsp70), adults of the millipede Julus scandinavius (Diplopoda) were exposed to leaf litter contaminated with different concentrations of Cd2+ (10, 30, 50 and 60 mg·kg-1 as CdCl2). The expression of hsp70 was investigated by semiquantitative and qualitative biochemical methods. After SDS-gel electrophoresis and Western blotting a subsequent digital image analysis showed that increasing dietary concentrations of Cd2+ resulted in elevated levels of hsp70, which in turn indicated proteotoxic condition. Qualitative results were obtained by two-dimensional gel electrophoresis. A stress-70 protein family, similar to that of other arthropods, was detected in Julus scandinavius: at least five different proteins with an approximate molecular weight of 68, 69, 70, 77, and 78 kDa could be distinguished after heat shock as well as after Cd2+ exposure.

Key words

Cadmium Stress-70 hsp70 Two-dimensional gel electrophoresis Diplopod, Julus scandinavius 

Abbreviations

IEF

isoelectric focusing

hsp

heat shock protein(s)

grp

glucose regulated protein(s)

PAGE

polyacrylamide gel electrophoresis

SDS

sodium dodecyl sulphate

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • M. Zanger
    • 1
  • G. Alberti
    • 1
  • M. Kuhn
    • 2
  • H. -R. Köhler
    • 1
  1. 1.Zoological Institute I (Morphology and Ecology)University of HeidelbergHeidelbergGermany
  2. 2.Department of Molecular GeneticsUniversity of HeidelbergHeidelbergGermany

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