Human Genetics

, Volume 35, Issue 1, pp 83–89

Laser UV microirradiation of interphase nuclei and post-treatment with caffeine

A new approach to establish the arrangement of interphase chromosomes
  • Christian Zorn
  • Thomas Cremer
  • Christoph Cremer
  • Jürgen Zimmer
Original Investigations

DOI: 10.1007/BF00295622

Cite this article as:
Zorn, C., Cremer, T., Cremer, C. et al. Hum Genet (1976) 35: 83. doi:10.1007/BF00295622

Summary

Laser UV microirradiation of Chinese hamster interphase cells combined with caffeine post-treatment produced different patterns of chromosome damage in mitosis following irradiation of a small area of the nucleus that may be classified in three categories: I) intact metaphase figures, II) chromosome damage confined to a small area of the metaphase spread, III) mitotic figures with damage on all chromosomes. Category III might be the consequence of a non-localized distortion of nuclear metabolism. By contrast, category II may reflect localized DNA damage induced by microirradiation, which could not be efficiently repaired due to the effect of caffeine. If this interpretation is right, in metaphase figures of category II chromosome damage should occur only at the irradiation site. The effect might then be used to investigate neighbourhood relationships of individual chromosomes in the interphase nucleus.

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Christian Zorn
    • 1
  • Thomas Cremer
    • 1
  • Christoph Cremer
    • 1
  • Jürgen Zimmer
    • 1
  1. 1.Institut für Humangenetik und Anthropologie der UniversitätFreiburg i. Br.Federal Republic of Germany