Current Microbiology

, Volume 31, Issue 1, pp 44–48

Photooxidative damage to the cyanobacterium Spirulina platensis mediated by singlet oxygen

  • D. P. Singh
  • Neeraj Singh
  • Kavita Verma
Article

DOI: 10.1007/BF00294633

Cite this article as:
Singh, D.P., Singh, N. & Verma, K. Current Microbiology (1995) 31: 44. doi:10.1007/BF00294633

Abstract

Experiments on the specific growth rate, bleaching of pigments, O2 evolution, lipid peroxidation, and loss of sulfhydryl (-SH) content in response to the varying light intensities (2–28 W/m2) suggested that photodamage to the Spirulina cells was maximum at or beyond the photosynthesis saturating light intensity (12 W/m2). However, photobleaching of the chlorophyll a was relatively higher than β carotenoid. The results on the N,N-dimethyl-p-nitrosoaniline (RNO) bleaching in the presence of oxygen radical quenchers exhibited maximum effect of sodium azide and indicated about the generation of singlet oxygen. The chlorophyll a-sensitized production of singlet oxygen by a type II reaction cannot be ruled out because of maximum oxidative damage to the cells at or beyond the photosynthesis saturating light intensity, i.e., 12 W/m2, when the availability of triplet chlorophyll is maximum.

Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • D. P. Singh
    • 1
  • Neeraj Singh
    • 2
  • Kavita Verma
    • 2
  1. 1.Department of MicrobiologyAvadh UniversityFaizabadIndia
  2. 2.Department of MicrobiologyBarkatullah UniversityBhopalIndia