Molecular and General Genetics MGG

, Volume 231, Issue 1, pp 7–16

Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase

Authors

  • Odile Ozier-Kalogeropoulos
    • Centre de Génétique Moléculaire du C.N.R.S.Laboratoire propre associé à l'Université Pierre et Marie Curie
  • Franco Fasiolo
    • I.B.M.C. du C.N.R.S.
  • Marie-Therèse Adeline
    • Institut de Chimie des Substances Naturelles du C.N.R.S.
  • Jocelyne Collin
    • Centre de Génétique Moléculaire du C.N.R.S.Laboratoire propre associé à l'Université Pierre et Marie Curie
  • François Lacroute
    • Centre de Génétique Moléculaire du C.N.R.S.Laboratoire propre associé à l'Université Pierre et Marie Curie
Article

DOI: 10.1007/BF00293815

Cite this article as:
Ozier-Kalogeropoulos, O., Fasiolo, F., Adeline, M. et al. Molec. Gen. Genet. (1991) 231: 7. doi:10.1007/BF00293815

Summary

The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5′-triphosphate to cytidine 5′-triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA 7 gene. The coding region is 1710 by long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate.

Key words

Saccharomyces cerevisiaeCTP synthetasePyrimidine pathwayIntracellular pyrimidine poolNon-essential gene

Copyright information

© Springer-Verlag 1991