Molecular and General Genetics MGG

, Volume 231, Issue 1, pp 7–16

Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase

  • Odile Ozier-Kalogeropoulos
  • Franco Fasiolo
  • Marie-Therèse Adeline
  • Jocelyne Collin
  • François Lacroute
Article

DOI: 10.1007/BF00293815

Cite this article as:
Ozier-Kalogeropoulos, O., Fasiolo, F., Adeline, MT. et al. Molec. Gen. Genet. (1991) 231: 7. doi:10.1007/BF00293815

Summary

The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5′-triphosphate to cytidine 5′-triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA 7 gene. The coding region is 1710 by long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate.

Key words

Saccharomyces cerevisiaeCTP synthetasePyrimidine pathwayIntracellular pyrimidine poolNon-essential gene

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Odile Ozier-Kalogeropoulos
    • 1
  • Franco Fasiolo
    • 2
  • Marie-Therèse Adeline
    • 3
  • Jocelyne Collin
    • 1
  • François Lacroute
    • 1
  1. 1.Centre de Génétique Moléculaire du C.N.R.S.Laboratoire propre associé à l'Université Pierre et Marie CurieGif-sur-Yvette CedexFrance
  2. 2.I.B.M.C. du C.N.R.S.Strasbourg CedexFrance
  3. 3.Institut de Chimie des Substances Naturelles du C.N.R.S.Gif-sur-Yvette CedexFrance