Article

Molecular and General Genetics MGG

, Volume 231, Issue 1, pp 7-16

First online:

Cloning, sequencing and characterization of the Saccharomyces cerevisiae URA7 gene encoding CTP synthetase

  • Odile Ozier-KalogeropoulosAffiliated withCentre de Génétique Moléculaire du C.N.R.S., Laboratoire propre associé à l'Université Pierre et Marie Curie
  • , Franco FasioloAffiliated withI.B.M.C. du C.N.R.S.
  • , Marie-Therèse AdelineAffiliated withInstitut de Chimie des Substances Naturelles du C.N.R.S.
  • , Jocelyne CollinAffiliated withCentre de Génétique Moléculaire du C.N.R.S., Laboratoire propre associé à l'Université Pierre et Marie Curie
  • , François LacrouteAffiliated withCentre de Génétique Moléculaire du C.N.R.S., Laboratoire propre associé à l'Université Pierre et Marie Curie

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Summary

The URA7 gene of Saccharomyces cerevisiae encodes CTP synthetase (EC 6.3.4.2) which catalyses the conversion of uridine 5′-triphosphate to cytidine 5′-triphosphate, the last step of the pyrimidine biosynthetic pathway. We have cloned and sequenced the URA 7 gene. The coding region is 1710 by long and the deduced protein sequence shows a strong degree of homology with bacterial and human CTP synthetases. Gene disruption shows that URA7 is not an essential gene: the level of the intracellular CTP pool is roughly the same in the deleted and the wild-type strains, suggesting that an alternative pathway for CTP synthesis exists in yeast. This could involve either a divergent duplicated gene or a different route beginning with the amination of uridine mono- or diphosphate.

Key words

Saccharomyces cerevisiae CTP synthetase Pyrimidine pathway Intracellular pyrimidine pool Non-essential gene