Human Genetics

, Volume 69, Issue 3, pp 233–237

New classes of common fragile sites induced by 5-azacytidine and bromodeoxyuridine

Authors

  • G. R. Sutherland
    • Cytogenetics Unit, Department of HistopathologyAdelaide Children's Hospital
  • M. I. Parslow
    • Department of Community Health, School of MedicineUniversity of Auckland
  • Elizabeth Baker
    • Cytogenetics Unit, Department of HistopathologyAdelaide Children's Hospital
Original Investigations

DOI: 10.1007/BF00293031

Cite this article as:
Sutherland, G.R., Parslow, M.I. & Baker, E. Hum Genet (1985) 69: 233. doi:10.1007/BF00293031

Summary

Two new classes of common fragile site seen in chromosomes from blood lymphocyte cultures are reproted. The first cláss is induced in bands 1q42 and 19q13 by 5-azacytidine (5-AZA). Maximum induction of these fragile sites occurs when the 5-AZA is added 5–8 h prior to harvest. The second class is induced in bands 6q13, 9p21, and 10q21 by bromodeoxyuridine (BrdU). In this instance maximum induction occurred if the BrdU was added 4–6h prior to harvest. The known fragile sites, both rare and common, are summarised.

Download to read the full article text

Copyright information

© Springer-Verlag 1985