Human Genetics

, Volume 69, Issue 3, pp 233–237

New classes of common fragile sites induced by 5-azacytidine and bromodeoxyuridine

  • G. R. Sutherland
  • M. I. Parslow
  • Elizabeth Baker
Original Investigations

DOI: 10.1007/BF00293031

Cite this article as:
Sutherland, G.R., Parslow, M.I. & Baker, E. Hum Genet (1985) 69: 233. doi:10.1007/BF00293031

Summary

Two new classes of common fragile site seen in chromosomes from blood lymphocyte cultures are reproted. The first cláss is induced in bands 1q42 and 19q13 by 5-azacytidine (5-AZA). Maximum induction of these fragile sites occurs when the 5-AZA is added 5–8 h prior to harvest. The second class is induced in bands 6q13, 9p21, and 10q21 by bromodeoxyuridine (BrdU). In this instance maximum induction occurred if the BrdU was added 4–6h prior to harvest. The known fragile sites, both rare and common, are summarised.

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • G. R. Sutherland
    • 1
  • M. I. Parslow
    • 2
  • Elizabeth Baker
    • 1
  1. 1.Cytogenetics Unit, Department of HistopathologyAdelaide Children's HospitalNorth AdelaideAustralia
  2. 2.Department of Community Health, School of MedicineUniversity of AucklandNew Zealand