Molecular and General Genetics MGG

, Volume 231, Issue 3, pp 385–394

Drosophila melanogaster paramyosin : developmental pattern, mapping and properties deduced from its complete coding sequence

  • Javier Vinós
  • Miguel Maroto
  • Rafael Garesse
  • Roberto Marco
  • Margarita Cervera
Article

DOI: 10.1007/BF00292707

Cite this article as:
Vinós, J., Maroto, M., Garesse, R. et al. Molec. Gen. Genet. (1992) 231: 385. doi:10.1007/BF00292707

Summary

Several cDNA clones encoding the complete Drosophila paramyosin sequence, including two potential polyadenylation sites, have been obtained. Southern analysis and in situ hybridization to polytene chromosomes indicate that in Drosophila the paramyosin gene is single copy, located on the left arm of the third chromosome at region 66D14. Northern analyses show predominantly two different RNAs which are the products of the choice between the two alternative polyadenylation sites. The two species begin to be synthesized around 10 h of development when embryonic muscles are formed, expression peaking at the end of embryogenesis. The protein is first expressed at germ band shortening in association with muscle precursor cells. A second maximum of paramyosin RNA expression occurs at late pupal stages when the higher molecular weight form becomes more abundant. In young adults this species becomes the main transcript detected. The 102 kDa polypeptide sequence is highly similar to that of Caenorhabditis elegans paramyosin. The protein has a central α-helical coiled-coil rod, organized in 29 groups of four typical seven-residue repeats and flanked by two short non-α-helical regions. Several leucine zippers are located on the hydrophobic face of the α-helix in paramyosin which, together with disulfide bonds between cysteines, are probably involved in the stabilization of the dimer. The structural and functional properties of Drosophila paramyosin deduced from the sequence are compared with those of known invertebrate myosins and paramyosins.

Key words

Drosophila Muscle proteins Paramyosin Leucine zippers Differential polyadenylation 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Javier Vinós
    • 1
  • Miguel Maroto
    • 1
  • Rafael Garesse
    • 1
  • Roberto Marco
    • 1
  • Margarita Cervera
    • 1
  1. 1.Departamento de Bioquímica de la UAM e Instituto de Investigaciones Biomédicas del CSICFacultad de Medicina de la Universidad Autónoma de MadridMadridSpain