Human Genetics

, Volume 69, Issue 4, pp 345–349

Isolation and characterisation of a cDNA clone for human apolipoprotein CI and assignment of the gene to chromosome 19

  • F. Tata
  • I. Henry
  • A. F. Markham
  • S. C. Wallis
  • D. Weil
  • K. H. Grzeschik
  • C. Junien
  • R. Williamson
  • S. E. Humphries
Article

DOI: 10.1007/BF00291654

Cite this article as:
Tata, F., Henry, I., Markham, A.F. et al. Hum Genet (1985) 69: 345. doi:10.1007/BF00291654

Summary

We have synthesised a mixed oligonucleotide 17 bases long and used it to isolate cDNA clones for apolipoprotein CI (apo CI) from an adult liver cDNA library. The partial sequence of one of these clones confirms its identity. We have used this probe and Southern blotting techniques to identify the human apo CI gene in DNA from a series of rodent x human somatic cell hybrids. Our Results provide evidence for the assignment of this gene to human chromosome 19.

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • F. Tata
    • 1
  • I. Henry
    • 2
  • A. F. Markham
    • 3
  • S. C. Wallis
    • 1
  • D. Weil
    • 4
  • K. H. Grzeschik
    • 5
  • C. Junien
    • 2
  • R. Williamson
    • 1
  • S. E. Humphries
    • 1
  1. 1.Department of BiochemistrySt. Mary's Hospital Medical SchoolLondonEngland UK
  2. 2.Groupe de Recherches de Biologie PrenataleINSERM U73, Chateau de LongchampParisFrance
  3. 3.Pharmaceutical DivisionImperial Chemical IndustriesMacclesfieldEngland, UK
  4. 4.Unite de Recherches de Genetique MedicaleINSERM, Centre Hospitalier Universitaire Necker-Enfants MaladeParis Cedex 15France
  5. 5.Institut für Humangenetik der UniversitätMünsterGermany