Article

Human Genetics

, Volume 69, Issue 4, pp 345-349

Isolation and characterisation of a cDNA clone for human apolipoprotein CI and assignment of the gene to chromosome 19

  • F. TataAffiliated withDepartment of Biochemistry, St. Mary's Hospital Medical School
  • , I. HenryAffiliated withGroupe de Recherches de Biologie Prenatale, INSERM U73, Chateau de Longchamp
  • , A. F. MarkhamAffiliated withPharmaceutical Division, Imperial Chemical Industries
  • , S. C. WallisAffiliated withDepartment of Biochemistry, St. Mary's Hospital Medical School
  • , D. WeilAffiliated withUnite de Recherches de Genetique Medicale, INSERM, Centre Hospitalier Universitaire Necker-Enfants Malade
  • , K. H. GrzeschikAffiliated withInstitut für Humangenetik der Universität
  • , C. JunienAffiliated withGroupe de Recherches de Biologie Prenatale, INSERM U73, Chateau de Longchamp
  • , R. WilliamsonAffiliated withDepartment of Biochemistry, St. Mary's Hospital Medical School
  • , S. E. HumphriesAffiliated withDepartment of Biochemistry, St. Mary's Hospital Medical School

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Summary

We have synthesised a mixed oligonucleotide 17 bases long and used it to isolate cDNA clones for apolipoprotein CI (apo CI) from an adult liver cDNA library. The partial sequence of one of these clones confirms its identity. We have used this probe and Southern blotting techniques to identify the human apo CI gene in DNA from a series of rodent x human somatic cell hybrids. Our Results provide evidence for the assignment of this gene to human chromosome 19.