Human Genetics

, Volume 69, Issue 4, pp 304–308

Regional chromosome mapping of human collagen genes alpha 2(I) and alpha 1(I) (COLIA2 and COLIA1)

  • E. Retief
  • M. I. Parker
  • A. E. Retief
Article

DOI: 10.1007/BF00291646

Cite this article as:
Retief, E., Parker, M.I. & Retief, A.E. Hum Genet (1985) 69: 304. doi:10.1007/BF00291646

Summary

For the assignment of the genes for the pro-α2(I) (COLIA2) and the pro-α1(I) (COLIA1) collagens, cDNA and genomic DNA probes were used in in situ hybridization experiments on human prometaphase chromosomes. An improved staining method is reported for the simultaneous identification of chromosomes and the autoradiographic grains after the hybridization procedures. With this procedure more cells with higher resolution could be used for the assignment of genes by in situ hybridization. Statistical analysis of the grains located on respectively 660 and 302 metaphases using pro-α2(I) and proα1(I) DNA probes, confirmed the assignment of these genes to human chromosomes 7 and 17. Analysis of the grain distribution on prometaphase chromosomes showed that the location of the proα2(I) collagen gene is in the region 7q21.3–22.1. The location of the pro-α1(I) collagen gene was found to be in band 17q21.31–2005.

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • E. Retief
    • 1
  • M. I. Parker
    • 2
  • A. E. Retief
    • 1
  1. 1.M. R. C. Research Unit for CytogeneticsUniversity of Stellenbosch Medical SchoolTygerbergRepublic of South Africa
  2. 2.Department Medical BiochemistryUniversity of Cape TownCape TownSouth Africa