Archives of Microbiology

, Volume 159, Issue 4, pp 316–322

Carbon source control on β-glucanases, chitobiase and chitinase from Trichoderma harzianum

  • Jesús de la Cruz
  • Manuel Rey
  • José M. Lora
  • Antonio Hidalgo-Gallego
  • Fernando Domínguez
  • José A. Pintor-Toro
  • Antonio Llobell
  • Tahía Benítez
Original Papers

DOI: 10.1007/BF00290913

Cite this article as:
de la Cruz, J., Rey, M., Lora, J.M. et al. Arch. Microbiol. (1993) 159: 316. doi:10.1007/BF00290913

Abstract

The cell-wall degrading enzymes β-glucanase and chitinase have been suggested to be essential for the mycoparasitic action of Trichoderma species against plant fungal pathogens. For this reason, the production in different carbon sources of extracellular β-1,3-glucanase, β-1,6-glucanase, chitobiase and chitinase was studied in a mycoparasitic strain of Trichoderma harzianum. Maximal β-glucanase specific activities were detected in media supplemented with either pustulan (β-1,6-glucan), nigeran (α-1,3-glucan alternating with α-1,4-glucan), chitin or Saccharomyces cerevisiae or Botrytis cinerea purified cell walls, whereas the highest chitinase specific activity was obtained in medium supplemented with chitin. Furthermore, β-glucanase, chitobiase and chitinase activities showed an increase parallel to increasing concentrations of either pustulan or chitin added to the cultures, although the extent of this increase varied with the different enzymes. The culture filtrates of T. harzianum grown in these carbon sources also showed lytic activity on purified cell walls of S. cerevisiae and B. cinerea. The enzyme synthesis seemed to be repressed by glucose, 8-hydroxyquinoline, which inhibits transcription, or cycloheximide, an inhibitor of protein synthesis.

Key words

ChitinaseChitobiaseBeta-glucanasesMycoparasitismTrichoderma

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Jesús de la Cruz
    • 1
  • Manuel Rey
    • 2
  • José M. Lora
    • 2
  • Antonio Hidalgo-Gallego
    • 2
  • Fernando Domínguez
    • 1
  • José A. Pintor-Toro
    • 3
  • Antonio Llobell
    • 1
  • Tahía Benítez
    • 2
  1. 1.Instituto de Bioquímica Vegetal y FotosíntesisCSIC-Universidad de SevillaSevillaSpain
  2. 2.Departamento de Genética, Facultad de BiologiaUniversidad de SevillaSevillaSpain
  3. 3.Instituto de Recursos Naturales y AgrobiologiaCSICSevillaSpain