Molecular and General Genetics MGG

, Volume 246, Issue 6, pp 716–722

Endosperm-specific demethylation and activation of specific alleles of α-tubulin genes of Zea mays L.

  • Gertrud Lund
  • Joachim Messing
  • Angelo Viotti
Original Paper

DOI: 10.1007/BF00290717

Cite this article as:
Lund, G., Messing, J. & Viotti, A. Molec. Gen. Genet. (1995) 246: 716. doi:10.1007/BF00290717

Abstract

We have investigated the methylation status of the α-tubulin genes, and the degree of accumulation of their mRNAs in endosperm, embryo and seedling tissues of Zea mays L. We have found that many of the α-tubulin genes are differentially demethylated in the endosperm relative to the embryo and seedling. However, only for tubα2 and tubα4 could a correlation between DNA demethylation and increased RNA accumulation be detected. By analyzing the inbred lines W64A and A69Y and their reciprocal crosses, we have also identified in the endosperm two α-tubulin genes, tubα3 and tubα4, that are differentially demethylated if transmitted by the maternal germline, but that remain hypermethylated when transmitted by the paternal germline.

Key words

α-tubulin geneDNA methylationEndospermParental imprintingZea mays

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Gertrud Lund
    • 1
    • 2
  • Joachim Messing
    • 1
  • Angelo Viotti
    • 2
  1. 1.Waksman Institute, RutgersThe State University of New JerseyPiscatawayUSA
  2. 2.Istituto Biosintesi Vegetali, CNRMilanoItaly