Original Paper

Molecular and General Genetics MGG

, Volume 249, Issue 2, pp 209-216

First online:

Yeast Kre1p is a cell surface O-glycoprotein

  • Terry RoemerAffiliated withBiology Department, McGill University
  • , Howard BusseyAffiliated withBiology Department, McGill University

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Abstract

The Saccharomyces cerevisiae KRE1 gene encodes a secretory protein required for the production of the cell wall polymer (1 → 6)-β-glucan. Here we report further characterization of the KRE1 gene product, Krelp. A functional, epitope-tagged Krelp is shown to be highly modified in a SEC53-dependent manner. Krelp is O-glycosylated, but the basis for the majority of its post-translational modification is unknown. Fractionation of Kre1p reveals a cell wall-associated form and a less abundant membrane-associated species. Indirect immunoflurorescence demonstrates that Kre1p localizes to the cell surface, where it becomes concentrated at the surface of mother cells. Such a localization of Kre1p seems to parallel the CAL1/CSD2-dependent cell wall deposition of chitin found in S. cerevisiae, and is consistent with evidence from Schizophyllum commune that (1 → 6)-β-glucan accumulates during maturation of the subapical region of the wall distal to the hyphal tip.

Key words

Yeast Cell wall β-Glucan synthesis Kre1p O-glycoprotein