Molecular and General Genetics MGG

, Volume 247, Issue 5, pp 583–590

A fungal actin-related protein involved in nuclear migration


  • Malcolm J. Robb
    • Department of BiologyCarleton University
  • Margaret A. Wilson
    • Department of BiologyCarleton University
  • P. John Vierula
    • Department of BiologyCarleton University
Original Paper

DOI: 10.1007/BF00290350

Cite this article as:
Robb, M.J., Wilson, M.A. & Vierula, P.J. Molec. Gen. Genet. (1995) 247: 583. doi:10.1007/BF00290350


The ro-4 mutant of the filamentous fungus Neurospora crassa forms distinctive colonies in which hyphae grow into rope-like aggregates. This unusual morphology coincides with a defect in hyphal nuclear migration. The ro-4 gene was cloned from a cosmid library by complementation of the closely linked pab-2 gene. The deduced 380 amino acid protein is most similar to the vertebrate actin-related protein/centractin. The R04 protein is not essential for cell viability, and new strains created by inducing point mutations at the ro-4 locus have a phenotype which is very similar to that of the original mutant. This study provides genetic evidence that an actin-related protein plays a role in nuclear motility. Since nuclear motility is believed to be a microtubule-dependent process, the ro-4 gene product may function as a component of the dynactin complex which activates force generation by cytoplasmic dynein.


Actin-related proteinNuclear migration CytoskeletonNeurospora
Download to read the full article text

Copyright information

© Springer-Verlag 1995