Original Paper

Molecular and General Genetics MGG

, Volume 249, Issue 1, pp 65-73

First online:

Combined mapping of AFLP and RFLP markers in barley

  • Jörg BeckerAffiliated withMax-Planck-Institut für Züchtungsforschung
  • , Pieter VosAffiliated withKeyGene N. V.
  • , Martin KuiperAffiliated withKeyGene N. V.
  • , Francesco SalaminiAffiliated withMax-Planck-Institut für Züchtungsforschung
  • , Manfred HeunAffiliated withMax-Planck-Institut für Züchtungsforschung

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Abstract

AFLP marker technology allows efficient DNA fingerprinting and the analysis of large numbers of polymorphic restriction fragments on polyacrylamide gels. Using the doubled haploids from the F1 of the cross Proctor × Nudinka, 118 AFLP markers were mapped onto a barley (Hordeum vulgare L.) RFLP map, also including five microsatellite and four protein marker loci. The AFLP markers mapped to all parts of the barley chromosomes and filled in the gaps on barley chromosomes 2L, 4L and 6 in which no RFLP loci had been mapped. Interestingly, the AFLP markers seldom interrupted RFLP clusters, but grouped next to them. The combined map covers 1873 cM, with a total of 282 markers. The merging of AFLP and RFLP markers increased the total map length; 402 cM were added to the map at the tips of chromosomes or in regions corresponding to earlier gaps. Another 375 cM resulted from mapping AFLP markers near to RFLP clusters or in between non-clustered RFLP markers.

Key words

Barley RFLP markers AFLP markers Genome mapping Genome mapping