Molecular and General Genetics MGG

, Volume 249, Issue 1, pp 65–73

Combined mapping of AFLP and RFLP markers in barley

  • Jörg Becker
  • Pieter Vos
  • Martin Kuiper
  • Francesco Salamini
  • Manfred Heun
Original Paper

DOI: 10.1007/BF00290237

Cite this article as:
Becker, J., Vos, P., Kuiper, M. et al. Molec. Gen. Genet. (1995) 249: 65. doi:10.1007/BF00290237

Abstract

AFLP marker technology allows efficient DNA fingerprinting and the analysis of large numbers of polymorphic restriction fragments on polyacrylamide gels. Using the doubled haploids from the F1 of the cross Proctor × Nudinka, 118 AFLP markers were mapped onto a barley (Hordeum vulgare L.) RFLP map, also including five microsatellite and four protein marker loci. The AFLP markers mapped to all parts of the barley chromosomes and filled in the gaps on barley chromosomes 2L, 4L and 6 in which no RFLP loci had been mapped. Interestingly, the AFLP markers seldom interrupted RFLP clusters, but grouped next to them. The combined map covers 1873 cM, with a total of 282 markers. The merging of AFLP and RFLP markers increased the total map length; 402 cM were added to the map at the tips of chromosomes or in regions corresponding to earlier gaps. Another 375 cM resulted from mapping AFLP markers near to RFLP clusters or in between non-clustered RFLP markers.

Key words

BarleyRFLP markersAFLP markers Genome mappingGenome mapping

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Jörg Becker
    • 1
  • Pieter Vos
    • 2
  • Martin Kuiper
    • 2
  • Francesco Salamini
    • 1
  • Manfred Heun
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKölnGermany
  2. 2.KeyGene N. V.AE WageningenThe Netherlands
  3. 3.Division of Population Genetics, Department Biotechnological SciencesAgricultural University NorwayAs-NLHNorway