Molecular and General Genetics MGG

, Volume 246, Issue 1, pp 56–64

Heat shock protein HSP60 can alleviate the phenotype of mitochondrial RNA-deficient temperature-sensitive mna2 pet mutants

Authors

  • Arunik Sanyal
    • Department of MedicineUniversity of Chicago
  • Alexis Harington
    • Centre de Genetique Moleculaire du C.N.R.S.Laboratoire propre associé a' l'Université Pierre et Marie Curie
  • Christopher J. Herbert
    • Centre de Genetique Moleculaire du C.N.R.S.Laboratoire propre associé a' l'Université Pierre et Marie Curie
  • Olga Groudinsky
    • Centre de Genetique Moleculaire du C.N.R.S.Laboratoire propre associé a' l'Université Pierre et Marie Curie
  • Piotr P. Slonimski
    • Centre de Genetique Moleculaire du C.N.R.S.Laboratoire propre associé a' l'Université Pierre et Marie Curie
  • Beatrice Tung
    • Department of PathologyUniversity of Chicago
  • Godfrey S. Getz
    • Department of MedicineUniversity of Chicago
    • Department of PathologyUniversity of Chicago
    • Department of Biochemistry and Molecular BiologyUniversity of Chicago
Original Paper

DOI: 10.1007/BF00290133

Cite this article as:
Sanyal, A., Harington, A., Herbert, C.J. et al. Molec. Gen. Genet. (1995) 246: 56. doi:10.1007/BF00290133

Abstract

mna2, which belongs to the class I temperature-sensitive pet mutants that lose mitochondrial (mt)RNA at restrictive temperature, was shown by complementation and sequence determination to correspond to the gene coding for HSP60. Both mna2-1 and mna2-2, the two available alleles of mna2, have conservative single amino acid substitutions in the HSP60 gene. Valine substitutes for an alanine (position 47) in mna2-1, and an isoleucine substitutes for a valine (position 77) in mna2-2. These substitutions result in defects in respiration and in steady-state mtRNA accumulation. Wild-type hsp60 alleviates the mtRNA phenotype completely, while partially relieving the respiratory deficiency.

Key words

Temperature-sensitive mutantsHeat shock protein 60Conservative single amino acid substitutionsMitochondrial biogenesisSaccharomyces cerevisiae

Copyright information

© Springer-Verlag 1995