Chromosoma

, Volume 94, Issue 3, pp 162–168

Centromeric DNA from Saccharomyces uvarum is functional in Saccharomyces cerevisiae

  • Joel A. Huberman
  • R. David Pridmore
  • Daniel Jäger
  • Ben Zonneveld
  • Peter Philippsen
Article

DOI: 10.1007/BF00288490

Cite this article as:
Huberman, J.A., Pridmore, R.D., Jäger, D. et al. Chromosoma (1986) 94: 162. doi:10.1007/BF00288490

Abstract

Previous comparisons of centromeric DNA sequences in laboratory strains of Saccharomyces cerevisiae have revealed conserved sequences within 120 base pairs (bp) which appear to be essential for centromere function. We wanted to find out whether centromeric DNA in Saccharomyces strains with different degrees of DNA sequence divergence carry the same conserved sequences or not. Bam HI DNA fragments from two S. cerevisiae strains and one Saccharomyces uvarum strain were cloned into a centromere selection vector and tested for centromere function in a S. cerevisiae laboratory strain. Fragments having centromere function were obtained at approximately equal frequencies from all three strains. Two of the S. uvarum centromeric DNAs and two of the S. cerevisiae centromeric DNAs were sequenced and shown to carry in a 120 bp region sequences essentially like those of centromeric DNA in S. cerevisiae laboratory strains. DNA hybridization to separated chromosomal DNAs revealed that the two newly determined S. cerevisiae centromeric DNA sequences belong to chromosomes V and XIII, respectively.

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Joel A. Huberman
    • 1
  • R. David Pridmore
    • 1
  • Daniel Jäger
    • 1
  • Ben Zonneveld
    • 1
  • Peter Philippsen
    • 1
  1. 1.Department of Microbiology, BiocenterUniversity of BaselBaselSwitzerland
  2. 2.Institut für Mikrobiologie und MolekularbiologieGießenFederal Republic of Germany