, Volume 65, Issue 2, pp 144-146

Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands

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The DA/DAPI technique has been found to be useful in the identification of specific chromosomal regions on human chromosomes. The realization that distamycin A (DA) is no longer commercially available has necessitated the development of an alternative technique. We describe a technique, MG/DAPI, which substitutes the AT-specific dye methyl green for distamycin A and gives results identical to those of the DA/DAPI technique.