Human Genetics

, Volume 82, Issue 2, pp 197–198

Demonstration of spontaneous XX/XY chimerism by DNA fingerprinting

  • C. M. Farber
  • M. Georges
  • G. De Bock
  • A. Verhest
  • P. Simon
  • M. Verschraegen-Spae
  • G. Vassart
Short Communications

DOI: 10.1007/BF00284058

Cite this article as:
Farber, C.M., Georges, M., De Bock, G. et al. Hum Genet (1989) 82: 197. doi:10.1007/BF00284058

Summary

The M13 bacteriophage probe, which makes possible the establishment of DNA fingerprints, was used to study a phenotypically normal woman with a 46XY karyotype and her twin brother. Identical fingerprints and positive hybridzation with a series of Y-specific probes were obtained on blood samples from the siblings. DNA from a skin biopsy of the woman yielded a clearly different pattern and displayed no Y-specific hybridization, indicating that she is a spontaneous chimera. This study illustrates the use of DNA fingerprinting as a powerful and simple aid to the diagnosis of natural chimerism.

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • C. M. Farber
    • 1
  • M. Georges
    • 2
  • G. De Bock
    • 3
  • A. Verhest
    • 4
  • P. Simon
    • 5
  • M. Verschraegen-Spae
    • 6
  • G. Vassart
    • 7
  1. 1.Department of ImmunologyCliniques universitaires ErasmeBrusselsBelgium
  2. 2.Department of Genetics, Ecole VétérinaireUniversité de LiègeLiègeBelgium
  3. 3.Department of Gynaecology and ObstetricsGenees-en Heelkundige InstituutVivoordeBelgium
  4. 4.Department of PathologyCliniques Universitaires ErasmeBrusselsBelgium
  5. 5.Department of Gynaecology and ObstetricsCliniques Universitaires EransmeBrusselsBelgium
  6. 6.Center of Medical GeneticsGent UniversiteitGentBelgium
  7. 7.Institute of Interdisciplinary Research and Department of GeneticsUniversité Libre de Bruxelles, Faculty of MedicineBrusselsBelgium