Molecular and General Genetics MGG

, Volume 225, Issue 1, pp 158–167

The mitochondrial genome of fission yeast: inability of all introns to splice autocatalytically, and construction and characterization of an intronless genome

  • Bernd Schäfer
  • Ana Maria Merlos-Lange
  • Carola Anderl
  • Franz Welser
  • Manfred Zimmer
  • Klaus Wolf
Article

DOI: 10.1007/BF00282654

Cite this article as:
Schäfer, B., Merlos-Lange, A.M., Anderl, C. et al. Molec. Gen. Genet. (1991) 225: 158. doi:10.1007/BF00282654

Summary

In this paper we report the inability of four group I introns in the gene encoding subunit I of cytochrome c oxidase (cox1) and the group II intron in the apocytochrome b gene (cob) to splice autocatalytically. Furthermore we present the characterization of the first cox1 intron in the mutator strain anar-14 and the construction and characterization of strains with intronless mitochondrial genomes. We provide evidence that removal of introns at the DNA level (termed DNA splicing) is dependent on an active RNA maturase. Finally we demonstrate that the absence of introns does not abolish homologous mitochondrial recombination.

Key words

Schizosaccharomyces pombeMitochondrial genomeIntron DNA excisionAutocatalytic RNA splicingRecombination

Abbreviations

cox1, cox2, cox3

genes encoding subunits 1, 2 and 3 of cytochrome

c

oxidase

cob

gene encoding apocytochrome b

cox1I1, cox1I2a, cox1I2b, cox1I3

introns in cox1

cox1Ix+/−

indicates the presence or absence of the intron either in the native gene or after intron DNA excision

cox1Ixδ

is a deletion in the intron leading to respiratory deficiency

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Bernd Schäfer
    • 1
  • Ana Maria Merlos-Lange
    • 2
  • Carola Anderl
    • 3
  • Franz Welser
    • 1
  • Manfred Zimmer
    • 1
  • Klaus Wolf
    • 1
  1. 1.Institut für Mikrobiologie und Weinforschung der Johannes Gutenberg-UniversitätMainzGermany
  2. 2.Europäisches PatentamtMünchenGermany
  3. 3.Max Planck-Institut für BiochemieMartinsriedGermany