Human Genetics

, Volume 74, Issue 3, pp 239–243

DNA finger printing by oligonucleotide probes specific for simple repeats

  • S. Ali
  • C. R. Müller
  • J. T. Epplen
Original Investigations

DOI: 10.1007/BF00282541

Cite this article as:
Ali, S., Müller, C.R. & Epplen, J.T. Hum Genet (1986) 74: 239. doi:10.1007/BF00282541

Summary

Interspersed simple repetitive DNA is a convenient genetic marker for analysis of restriction fragment length polymorphisms (RFLPs) because of the numbers and the frequencies of its alleles. Oligonucleotide probes specific for variations of the GACTA simple repeats have been designed and hybridized to a panel of human DNAs digested with various restriction enzymes. Numerous RFLPs were demonstrated in AluI and MboI digested DNA with “pure” GATA oligonucleotides as probes. The optimal length of the probe for RFLP analysis was 20 bases taking into account fragment lengths (1.5-7 kilobases = kb), signal to background ratio, and number of clearly evaluable RFLPs. By using different restriction enzymes individual-specific hybridization patterns (“DNA fingerprints”) can be established. Hypervariable simple repeat fragments are stably inherited in a Mendelian fashion. Advantages of this method are discussed.

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • S. Ali
    • 1
  • C. R. Müller
    • 2
  • J. T. Epplen
    • 1
  1. 1.Junior Research UnitMax-Planck-Institut für ImmunbiologieFreiburgFederal Republic of Germany
  2. 2.Institut für Humangenetik der UniversitätWürzburgFederal Republic of Germany