Human Genetics

, Volume 73, Issue 4, pp 320–326

A routine method for the establishment of permanent growing lymphoblastoid cell lines

  • Heidemarie Neitzel
Original Investigations

DOI: 10.1007/BF00279094

Cite this article as:
Neitzel, H. Hum Genet (1986) 73: 320. doi:10.1007/BF00279094


Permanent lymphoblastoid cell lines are of great practical value in human clinical and experimental genetics. A detailed protocol for routine use is given for the establishment of lymphoblastoid lines from peripheral blood using Esptein-Barr virus and the immunosuppressivum Cyclosporin A. In addition, the biologic basis of this transformation system is briefly summarized.

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Heidemarie Neitzel
    • 1
  1. 1.Institut für HumangenetikFreie Universität BerlinBerlin-West 19Germany