Human Genetics

, Volume 54, Issue 1, pp 107–110

Detection of laser-UV microirradiation-induced DNA photolesions by immunofluorescent staining

Authors

  • C. Cremer
    • Institut für Humangenetik und AnthropologieUniversität Freiburg
  • T. Cremer
    • Institut für Anthropologie und HumangenetikUniversität Heidelberg
  • M. Fukuda
    • Department of PathologyKyoto Prefectural University of Medicine
  • K. Nakanishi
    • Department of PathologyKyoto Prefectural University of Medicine
Original Investigations

DOI: 10.1007/BF00279058

Cite this article as:
Cremer, C., Cremer, T., Fukuda, M. et al. Hum Genet (1980) 54: 107. doi:10.1007/BF00279058

Summary

A low-power laser-UV microbeam of wave-length 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light.

The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.

Copyright information

© Springer-Verlag 1980